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Sangon Biotech primers
Primers, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer/primers/pmc13264189-147-30-31
Average 86 stars, based on 1 article reviews
primers - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Countercurrent Chromatography:

Article Title: miR-133b-3p Mitigates D-Galactose-Induced Hippocampal Neuron Aging Through Autophagy Regulation via the MAPK/ERK Signaling Pathway.
Article Snippet: Although remarkable progress has been achieved in contemporary medical research, effective drugs or prophylactic approaches targeting neurodegenerative diseases associated with aging are still limited.. Increasing evidence suggests that microRNAs (miRNAs) are closely associated with age-related neurological diseases, positioning them as novel therapeutic targets.. Autophagy in neurons participates in the renewal of damaged or aged endoplasmic reticulum, mitochondria, other organelles, and aggregated proteins during aging.

Synthesized:

Article Title: miR-133b-3p Mitigates D-Galactose-Induced Hippocampal Neuron Aging Through Autophagy Regulation via the MAPK/ERK Signaling Pathway.
Article Snippet: Although remarkable progress has been achieved in contemporary medical research, effective drugs or prophylactic approaches targeting neurodegenerative diseases associated with aging are still limited.. Increasing evidence suggests that microRNAs (miRNAs) are closely associated with age-related neurological diseases, positioning them as novel therapeutic targets.. Autophagy in neurons participates in the renewal of damaged or aged endoplasmic reticulum, mitochondria, other organelles, and aggregated proteins during aging.

Article Title: Selection and characterization of novel DNA aptamers targeting colorectal cancer cells with malignant potential.
Article Snippet: Colorectal cancer (CRC) is associated with high morbidity and mortality rates worldwide.. Therefore, early diagnosis and treatment are of great significance for improving the survival rate of CRC patients.. Herein, we performed subtractive cellSELEX using two cell lines (LS174T and CL187) with the same genetic background but different malignant potentials for the target and negative cells.

Article Title: Aptamer based lateral flow biosensor for rapid detection of largemouth bass virus
Article Snippet: .. All aptamers, probes and primer were listed in , and synthesized by Sangon Biotech. ..

Polymerase Chain Reaction:

Article Title: Characterization of the complete mitochondrial genome of Longicollum pagrosomi yamaguti, 1935 (Palaeacanthocephala: Echinorhynchida) in cultured large yellow croaker ( Larimichthys crocea ) and its phylogenetic implications
Article Snippet: .. The PCR products were sequenced using a primer-walking strategy at Sangon Biotech. .. The mitogenomic sequences were manually assembled using DNASTAR v7.1 software (Burland, ) and annotated according to the procedures described by Li et al. ( ).

Article Title: Phenotypic and genotypic characterization of ST103 serotype Ia Streptococcus agalactiae isolated from bovine mastitis in China
Article Snippet: .. The PCR reactions consisted of 2 × Green Taq Mix (Vazyme, Nanjing, China) and 250 nM of each specific primer, except primers cps-Ia-6–7-F and cps-7–9-F, which used 400 nM of each (Shanghai Sangon Biotech Company, Shanghai, China). ..

Chromosome Walking:

Article Title: Characterization of the complete mitochondrial genome of Longicollum pagrosomi yamaguti, 1935 (Palaeacanthocephala: Echinorhynchida) in cultured large yellow croaker ( Larimichthys crocea ) and its phylogenetic implications
Article Snippet: .. The PCR products were sequenced using a primer-walking strategy at Sangon Biotech. .. The mitogenomic sequences were manually assembled using DNASTAR v7.1 software (Burland, ) and annotated according to the procedures described by Li et al. ( ).



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miR-151-3p is a key functional cargo in Exe-Exos mediating anti-apoptotic and antioxidant effects. (A – B) Differentially expressed <t>miRNAs</t> between Exe-Exos and Sed-Exos groups (q < 0.05, |log 2 FC| > 1). (C) RT-qPCR validation of 10 differentially expressed miRNAs. (D) Flow cytometry analysis of apoptosis in OGD/R, OGD/R + Exos, and OGD/R + Exos + miR-151-3p groups. (E) Quantification of apoptotic cell percentage (n = 3 independent cell culture experiments). (F) LDH activity (U/L; n = 5 independent cell culture experiments). (G) WB and (H – M) quantification of p-JNK, JNK, C-Caspase9, Caspase9, C-Caspase3, and Caspase3 protein expression (n = 6 independent biological replicates). Exos exosomes, JNK c-Jun N-terminal kinase.
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miR-151-3p is a key functional cargo in Exe-Exos mediating anti-apoptotic and antioxidant effects. (A – B) Differentially expressed miRNAs between Exe-Exos and Sed-Exos groups (q < 0.05, |log 2 FC| > 1). (C) RT-qPCR validation of 10 differentially expressed miRNAs. (D) Flow cytometry analysis of apoptosis in OGD/R, OGD/R + Exos, and OGD/R + Exos + miR-151-3p groups. (E) Quantification of apoptotic cell percentage (n = 3 independent cell culture experiments). (F) LDH activity (U/L; n = 5 independent cell culture experiments). (G) WB and (H – M) quantification of p-JNK, JNK, C-Caspase9, Caspase9, C-Caspase3, and Caspase3 protein expression (n = 6 independent biological replicates). Exos exosomes, JNK c-Jun N-terminal kinase.

Journal: Bioactive Materials

Article Title: Exercise-derived exosomal miR-151-3p: An innovative anti-inflammatory and antioxidant therapeutic for spinal cord injury

doi: 10.1016/j.bioactmat.2026.06.009

Figure Lengend Snippet: miR-151-3p is a key functional cargo in Exe-Exos mediating anti-apoptotic and antioxidant effects. (A – B) Differentially expressed miRNAs between Exe-Exos and Sed-Exos groups (q < 0.05, |log 2 FC| > 1). (C) RT-qPCR validation of 10 differentially expressed miRNAs. (D) Flow cytometry analysis of apoptosis in OGD/R, OGD/R + Exos, and OGD/R + Exos + miR-151-3p groups. (E) Quantification of apoptotic cell percentage (n = 3 independent cell culture experiments). (F) LDH activity (U/L; n = 5 independent cell culture experiments). (G) WB and (H – M) quantification of p-JNK, JNK, C-Caspase9, Caspase9, C-Caspase3, and Caspase3 protein expression (n = 6 independent biological replicates). Exos exosomes, JNK c-Jun N-terminal kinase.

Article Snippet: The miRNA stem-loop primers (Shanghai Jierui Bioengineering) are listed in .

Techniques: Functional Assay, Quantitative RT-PCR, Biomarker Discovery, Flow Cytometry, Cell Culture, Activity Assay, Expressing